Muscle Strip Myograph
Muscle Strip Myograph - Related Videos
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
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Cited by 8 •
2012
Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
07:25
Assessing Murine Resistance Artery Function Using Pressure Myography
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Cited by 18 •
2013
In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
11:48
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
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Cited by 5 •
2016
This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.
08:42
Myo-mechanical Analysis of Isolated Skeletal Muscle
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Cited by 25 •
2011
To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.
10:26
Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology
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Cited by 36 •
2014
This manuscript presents a simple, yet powerful, in vitro method for evaluating smooth muscle contractility in response to pharmacological agents or nerve stimulation. Main applications are drug screening and understanding tissue physiology, pharmacology, and pathology.
FAQs
The fixed support keeps one end of the strip stable while the force transducer detects tension generated at the other end. Recording that force over time converts contraction and relaxation into measurable changes rather than relying only on visual observation. This arrangement lets investigators compare how strongly and how consistently a tissue responds under defined experimental conditions.
Conditions in the bath provide the controlled environment in which the isolated tissue is tested. Adjusting those conditions can alter the tissue’s contractile behavior, so researchers can examine responsiveness under selected experimental settings. Because the system records tension continuously, changes associated with contraction or relaxation can be related to the applied condition rather than inferred from appearance alone.
Electrical stimulation and pharmacological agents probe muscle responsiveness in different ways. Stimulation tests the tissue’s contractile response when an electrical input is applied, whereas an agent tests how exposure to a substance changes tension or relaxation. Using these interventions in the same controlled setup helps distinguish general contractile behavior from responses associated with a particular experimental treatment.
An experiment begins by securing the isolated strip between a fixed support and the force transducer, then placing it in the controlled bath. Researchers adjust the experimental conditions, apply electrical stimulation or a pharmacological agent, and record tension over time. The resulting trace provides a direct record of the strip’s contractile response during the test.
Measurements can show the magnitude and time course of tension changes, along with whether the tissue contracts or relaxes in response to stimulation or an agent. These observations support characterization of muscle responsiveness and provide a quantitative basis for comparing experimental conditions or drug effects. The recorded changes can therefore describe both contractile behavior and treatment-related effects.
The technique allows investigators to examine contractile mechanisms in smooth, skeletal, or cardiac muscle and to evaluate therapies that affect those tissues. Controlled measurements can also support research on disorders involving abnormal muscle function. By recording changes in force and responsiveness, the system helps characterize medically relevant tissue behavior under defined experimental conditions.