Cell Recellularization
Cell Recellularization - Related Videos
02:27
Confirming Viral Transduction in Recellularized Rat Liver Using Immunohistochemical Staining
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2026
Source: Hiller, T. et. al. Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2. J. Vis. Exp. (2016)This video demonstrates immunohistochemical staining to evaluate viral gene transfer in a recellularized rat liver model. Reporter expression, confirmed by antibody detection and co-localization with nuclear staining, indicates successful viral transduction in the cells.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
11:35
Decellularization and Recellularization Methodology for Human Saphenous Veins
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Cited by 21 •
2018
Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.
16:45
Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
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Cited by 46 •
2013
Whole-organ decellularization produces natural biological scaffolds that may be used for regenerative medicine. The description of a nonhuman primate model of lung regeneration in which whole lungs are decellularized and then seeded with adult stem cells and endothelial cells in a bioreactor that facilitates vascular circulation and liquid media ventilation is presented.
09:13
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
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Cited by 7 •
2016
The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.
FAQs
The retained extracellular matrix does more than provide physical support: its architecture and biochemical cues create a framework that can influence how introduced cells attach, survive, and distribute. Because these features remain after native cells are removed, researchers can examine cellular behavior within a structured tissue-like environment rather than interpreting responses only from isolated cellular systems.
Cell choice and culture conditions are central determinants of successful repopulation. The selected cells must encounter conditions that support attachment and survival, while the introduction method must promote their distribution throughout the scaffold. These variables affect whether the resulting construct contains a useful cellular arrangement for studying tissue behavior or cancer-related interactions.
Tumor cells provide a cancer-relevant population, whereas stromal cells help represent the surrounding microenvironment. Combining them allows researchers to investigate interactions between malignant cells, supporting cells, and the extracellular matrix. This arrangement can reveal how the tissue framework contributes to invasion and other behaviors that may be difficult to evaluate in tumor cells alone.
A three-dimensional scaffold preserves spatial organization that can affect how cells contact the matrix and one another. This structure supports investigations of invasion and cellular responses within a more physiologically relevant setting. In cancer research, such organization helps connect observed behavior to the tissue framework, rather than treating matrix interactions as separate from the surrounding environment.
The workflow begins with removal of native cells while retaining the extracellular matrix architecture and biochemical cues. Researchers then introduce selected cells by seeding or through perfusion-based culture. Subsequent culture conditions are chosen to support attachment, survival, and distribution, producing a scaffold suitable for examining tumor, stromal, and cell–matrix behavior.
Both seeding and perfusion-based culture are approaches for introducing cells into a decellularized scaffold, but their practical value depends on the desired cellular distribution and culture setup. The source material identifies distribution as a key outcome supported by these methods. Researchers therefore select the approach that best supports cell placement, attachment, and survival within the matrix.
These models can be used to investigate tumor microenvironments, tumor invasion, cell–matrix interactions, and responses to therapies. Their three-dimensional structure may improve the physiological relevance of preclinical studies compared with less tissue-like systems. When tumor-related cells are selected appropriately, the platform can also support research into treatment strategies tailored to individual cancer contexts.