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Bioengineering

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JoVE Journal Bioengineering
Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
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Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Article DOI: 10.3791/50825 • 16:45 min
December 15th, 2013 •
1,2, 1, 1, 1, 1, 1,3, 1,3, 1,2,4
1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 2Division of Regenerative Medicine, Tulane National Primate Research Center, 3Department of Microbiology and Immunology, Tulane University School of Medicine, 4Department of Pharmacology, Tulane University School of Medicine

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December 15th, 2013

Whole-organ decellularization produces natural biological scaffolds that may be used for regenerative medicine. The description of a nonhuman primate model of lung regeneration in which whole lungs are decellularized and then seeded with adult stem cells and endothelial cells in a bioreactor that facilitates vascular circulation and liquid media ventilation is presented.

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