Murine Colon Isolation
Murine Colon Isolation - Related Videos
08:52
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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Cited by 8 •
2011
A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
02:48
Colonoscopic Guided Murine Wound Model: A Method to Isolate Biopsy Induced Wound Bed from Colon to Understand the Healing Process
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2025
In this video, we describe a procedure to isolate the colonoscopic-induced wound bed at an appropriate time point for detailed molecular analysis. This analysis helps to understand the wound healing process in inflammatory conditions of the GI tract.
06:16
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
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Cited by 5 •
2015
Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.
09:48
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
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Cited by 9 •
2019
The goal of this protocol is to isolate mononuclear cells that reside in the lamina propria of the colon by enzymatic digestion of the tissue using collagenase. This protocol allows for the efficient isolation of mononuclear cells resulting in a single cell suspension which in turn can be used for robust immunophenotyping.
FAQs
Orientation primarily preserves the positional context needed for reliable comparison between samples. Maintaining the cecum-to-rectum axis helps investigators relate lesions or tissue changes to defined colonic regions, while consistent sampling reduces variation between animals. This matters in cancer studies because apparent differences in tumor distribution or pathology may otherwise reflect handling or location rather than biology.
Processing format determines which biological questions can be answered. An intact segment retains tissue architecture for lesion mapping and histopathology, whereas dissociation separates epithelial, stromal, and immune populations for cell-focused analyses or downstream culture. Choosing between these formats therefore affects whether the experiment emphasizes spatial relationships or cellular composition.
Removing surrounding tissue and luminal contents improves interpretability by limiting material that could obscure the colon or interfere with downstream examination. Preservation must also match the intended analysis: structural studies depend on maintaining architecture, while molecular or cellular work depends on retaining suitable tissue or populations. Inconsistent clearing or preservation can complicate comparisons across experimental groups.
The colon is traced within the gastrointestinal tract from the cecum toward the rectum, then separated and cleared of attached tissue and contents. Maintaining this direction during collection supports consistent orientation and segment identification. Reproducible handling is especially important when comparing tumor location, burden, or tissue features across genetically engineered or chemically induced models.
In cancer research, the collected tissue supports complementary measurements of tumor burden and tissue pathology. Investigators can examine how extensively lesions affect the colon, then use histopathology to characterize associated structural changes. Applying the same collection and sampling approach across genetically engineered and chemically induced models makes model phenotypes more comparable and strengthens interpretation of disease progression.
Colon material can support molecular and functional follow-up. Gene and protein analyses identify disease-associated changes, while dissociated populations or suitable tissue preparations can support organoid culture. These readouts connect alterations in epithelial, stromal, or immune compartments with broader cancer biology, helping distinguish tissue composition, molecular state, and growth behavior as separate experimental outcomes.
Collected colons provide tissue for assessing treatment-associated changes in tumor burden, histopathology, gene expression, and protein patterns. If tumors persist or tissue changes remain despite therapy, these findings can be related to therapeutic resistance. Combining structural, molecular, and cellular endpoints helps distinguish reduced tumor size from deeper biological responses to treatment.