Human Dendritic Cells
Human Dendritic Cells - Related Videos
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
10:18
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
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Cited by 7 •
2015
Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.
09:04
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
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Cited by 7 •
2014
Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
08:31
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Cited by 19 •
2016
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
FAQs
After endocytosis, human dendritic cells process proteins into peptide fragments and display those fragments on major histocompatibility complex molecules. This processing converts captured pathogen or cellular material into antigenic information that T cells can inspect through their receptors. Studying this sequence helps researchers connect antigen capture with the specificity of later adaptive immune responses.
Maturation changes the functional state of dendritic cells so they can leave the site where material was encountered and migrate to lymphoid tissues. There, antigen display is paired with receptor and costimulatory signals that support T-cell activation. This makes maturation important when evaluating whether threat detection produces an effective targeted response rather than antigen capture alone.
Receptor recognition and costimulatory signals work alongside antigen display to activate antigen-specific T cells. Their combined presence links the identity of a captured antigen with the cellular signals needed for an adaptive response. In immunology studies, examining these signals helps clarify how dendritic cells shape targeted immunity after encountering pathogens or cellular material.
Dendritic cells connect early threat detection with a later antigen-specific response. They sense and capture pathogens or cellular material, process it for presentation, and, after maturation, provide signals that engage T cells. This bridging function is especially relevant to infection research because it helps explain how initial host recognition can lead to protective or pathological inflammation.
Characterizing dendritic-cell function can reveal how infections influence antigen capture, processing, maturation, migration, and T-cell activation. These measurements help investigators examine host-pathogen interactions and determine how immune responses develop after exposure. The resulting information can clarify why an infection supports protective immunity in some settings but contributes to pathological inflammation in others.
Their ability to process antigen and activate antigen-specific T cells makes human dendritic cells relevant to vaccine research. Investigators can use them to study how vaccine-associated material is converted into signals for adaptive immunity. This provides a cellular context for evaluating whether an approach can support a targeted immune response rather than simply trigger initial threat detection.
They provide a cellular point of connection between pathogens and the adaptive immune system. Researchers can examine how pathogen material is captured, processed, displayed, and linked to T-cell activation, while also assessing maturation and migration. These observations help define how infections shape immune communication and whether resulting inflammation is protective or pathological.
Dendritic-cell studies can be applied to questions about immune tolerance and cancer immunotherapy because these cells determine how cellular material is processed and presented to T cells. Characterizing their antigen-display and activation functions helps researchers investigate how immune responses are directed, restrained, or redirected. The same framework supports research into treatment strategies involving targeted adaptive immunity.