Liver Histology
Liver Histology - Related Videos
01:27
Liver Histology
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2024
The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria. Hepatocytes perform a variety of essential functions. They secrete...
10:45
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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Cited by 27 •
2017
This protocol describes histological analyses of the livers from zebrafish larvae that have been treated with 2% ethanol for 24 h. Such acute ethanol treatment results in hepatic steatosis and swelling of the hepatic vasculature.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
FAQs
The lobular arrangement organizes hepatocyte plates around a central vein, placing these cells alongside sinusoids that transport blood through the tissue. This spatial relationship supports the liver’s metabolic, detoxification, bile-producing, and blood-filtering activities. Examining the arrangement also helps identify architectural changes associated with hepatic disease.
Blood travels through sinusoids from portal triads toward the central vein, whereas bile moves through canaliculi toward bile ducts. These opposing pathways separate the movement of blood from the removal of bile. Their direction and relationship to hepatocytes are important features when interpreting microscopic liver organization and tissue abnormalities.
Assessment focuses on the organization of hepatocyte plates, central veins, sinusoids, portal triads, canaliculi, and bile ducts. Alterations in these structures can provide evidence of inflammation, fibrosis, fatty liver disease, cirrhosis, or tumors. The observed pattern helps connect microscopic tissue changes with the underlying hepatic condition.
Different diseases can produce different changes in liver tissue architecture and cellular organization. Histological examination may reveal patterns associated with inflammation, fibrosis, fatty accumulation, cirrhosis, or tumors. Recognizing these changes allows tissue findings to contribute to diagnosis and disease staging rather than relying only on the liver’s normal structural pattern.
A liver biopsy provides tissue for microscopic examination, allowing investigators to assess cellular organization and the condition of the lobular structures. The resulting observations can identify disease-related changes and support diagnosis. In clinical practice, this information also contributes to staging and helps inform treatment decisions for hepatic disorders.
Clinicians use microscopic liver analysis to support diagnosis, determine disease stage, and guide treatment decisions in conditions such as inflammation, fibrosis, fatty liver disease, cirrhosis, and tumors. In research, the same structural assessment helps investigate hepatic function and regeneration, linking tissue organization with the liver’s capacity to perform and restore its activities.