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A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
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A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

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12,097 Views

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08:04 min

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March 13, 2014

DOI:

10.3791/51103-v

DOI:
10.3791/51103-v

•

08:04 min
March 13, 2014

•

1 Views
, , ,
1Institute of Plant Biology and Biotechnology,University of Münster, 2Department of Plant Biology,Carnegie Institution for Science

Summary

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The described comparative, quantitative proteomic approach aims at obtaining insights into the composition of multiprotein complexes under different conditions and is demonstrated by comparing genetically different strains. For quantitative analysis equal volumes of different fractions from a sucrose density gradient are mixed and analyzed by mass spectrometry.

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Trompelt, K., Steinbeck, J., Terashima, M., Hippler, M. A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry. J. Vis. Exp. (85), e51103, doi:10.3791/51103 (2014).

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