Using protein microarrays containing nearly the entire S. cerevisiae proteome is probed for rapid unbiased interrogation of thousands of protein-protein interactions in parallel. This method can be utilized for protein-small molecule, posttranslational modification, and other assays in high-throughput.
Fasolo, J., Im, H., Snyder, M. P. Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions. J. Vis. Exp. (102), e51872, doi:10.3791/51872 (2015).