Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Joseph Fasolo; Hogune Im; Michael P. Snyder

doi:10.3791/51872

Journal

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Biology

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

JoVE Journal
Biology

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JoVE Journal Biology

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

7,959 Views

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08:07 min

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August 02, 2015

DOI:

10.3791/51872-v

DOI:

10.3791/51872-v

•

08:07 min

August 02, 2015

•

3 Views

Joseph Fasolo¹, Hogune Im¹, Michael P. Snyder^1,2

¹Department of Genetics,Stanford University, ²Stanford Center for Genomics and Personalized Medicine,Stanford University

Summary

Using protein microarrays containing nearly the entire S. cerevisiae proteome is probed for rapid unbiased interrogation of thousands of protein-protein interactions in parallel. This method can be utilized for protein-small molecule, posttranslational modification, and other assays in high-throughput.