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Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
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Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

DOI:
10.3791/1562-v

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09:37 min

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August 31, 2009

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, , , , , , , , , , ,
1Departments of Neurology,Columbia University, 2Departments of Psychiatry and Pharmacology,Columbia University, 3Department of Chemistry,Columbia University, 4eMolecules, Inc., 5Departments of Neurology and Physiology,University of California School of Medicine, San Francisco, 6Division of Molecular Therapeutics,New York Psychiatric Institute

Chapters

  • 00:06Title
  • 01:15Introduction
  • 01:41Preparing Acute Striatal Slices and Loading FFN511
  • 03:59Imaging FFN511 in the Brain Slices
  • 05:43FFN511 Destaining
  • 07:03Results of FFN511 Loading and Destaining in Brain Slices
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

A new means to measure neurotransmission optically using fluorescent dopamine analogs.

Tags

Dopamine ReleaseIndividual Presynaptic TerminalsFFNsNeurotransmitter UptakeSynaptic Vesicle FusionFluorescent False NeurotransmittersSynaptic PlasticityStimulus FrequencyReserve Synaptic Vesicle PopulationD2 Dopamine ReceptorsFrequency-dependent CodingPresynaptic Selection

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