Soybean Seed Sterilization

doi:10.3791/200164

Journal

/

Bioengineering

/

Soybean Seed Sterilization

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Soybean Seed Sterilization

585 Views

•

01:11 min

•

May 05, 2023

DOI:

10.3791/200164-v

DOI:

10.3791/200164-v

•

01:11 min

May 05, 2023

•

8 Views

Jie Lin¹, Dasol Wi¹, Melissa Ly¹, Md Asraful Jahan², Sarah Pullano¹, Izabella Martirosyan¹, Nik Kovinich¹

¹Department of Biology,York University, ²Department of Genetic Engineering and Biotechnology,Shahjalal University of Science and Technology

Transcript

To begin, place 16 to 20 pristine round Williams 82 soybean seeds in a 50 milliliter centrifuge tube inside a biosafety cabinet. Add 30 milliliters of 70%isopropyl alcohol to the tube and shake for 30 seconds. Decant the alcohol.

Next, gently shake the seeds with 30 milliliters of 10%bleach for 10 seconds, and allow the seeds to sit in the solution for 5 minutes at room temperature. After 5 minutes, drain the bleach then rinse the seeds three times with 30 milliliters of sterile ultrapure water for 1 minute per rinse and discard the water between each rinse. Place the sterilized seeds on filter paper saturated with 5 milliliters of germination and co-cultivation medium in a sterile petri dish.

Place the plates in the dark at room temperature for 3 days before transferring them at 22 degrees Celsius under 16 hour cool white T5 fluorescent lights for 4 days to allow the seeds to germinate.