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Investigating Zinc Transport Mechanism in Mammalian Cells with FluoZin-3 Assay
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染色トランスフェクションHEK293T細胞のサンプル調製と蛍光測定
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JoVE Journal Biology
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells
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染色トランスフェクションHEK293T細胞のサンプル調製と蛍光測定

Investigating Zinc Transport Mechanism in Mammalian Cells with FluoZin-3 Assay

DOI:
10.3791/200360-v

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04:55 min

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June 02, 2023

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, ,
1Department of Life Sciences and National Institute for Biotechnology in the Negev,Ben-Gurion University, 2Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University

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Keywords: Sample PreparationFluorescence MeasurementTransfected HEK293T CellsMicroscopeLight SourceCameraSuction SystemRinger’s SolutionRinger’s Zinc SolutionPyrithionePerfusion ChamberSealing SiliconeCoverslipRinger’s Washing SolutionPerfusion RateImaging SoftwareMCherryROIsBackground ROIGFPPerfect Focus SystemPFS

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