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Journal
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Developmental Biology
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Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis
/
FACSソートマウスサテライト細胞のヌクレアーゼ解析を用いたターゲット下での切断と放出
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Cleavage Under Targets and Release Using Nuclease Analysis of FACS-Sorted Mouse Satellite Cells
Please note that all translations are automatically generated.
Click here for the English version.
FACSソートマウスサテライト細胞のヌクレアーゼ解析を用いたターゲット下での切断と放出
Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis
DOI:
10.3791/200376-v
•
04:25 min
•
July 07, 2023
•
Kamar Ghaibour*
1
,
Joe Rizk*
1
,
Claudine Ebel
,
Tao Ye
,
Muriel Philipps
,
Valérie Schreiber
,
Daniel Metzger
,
Delphine Duteil
1
CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258
,
Université de Strasbourg
Tags
Keywords: Satellite Cells
FACS
Nuclear Extraction
Concanavalin A
Magnetic Beads
Binding Buffer
Wash Buffer
Micrococcal Nuclease
Calcium Chloride
Phenol:chloroform:isoamyl Alcohol
Proteinase K
SDS
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