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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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基于Liberase的酶法纯化从小鼠脊髓中分离的小胶质细胞

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Neuroscience

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JoVE Journal Neuroscience

Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord

Please note that all translations are automatically generated. Click here for the English version.

基于Liberase的酶法纯化从小鼠脊髓中分离的小胶质细胞

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

DOI:

10.3791/200616-v

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02:42 min

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September 22, 2023

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Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children’s Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

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Keywords: Microglia Isolation Spinal Cord Enzymatic Digestion Liberase Density Gradient Cell Purification FACS Analysis

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