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Harnessing iPSC-Derived iTenocytes for Effective Cell-Based Therapy

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주기적 스트레칭을 위한 2D 바이오리액터에서 iMSC^SCX+ 의 기계적 로딩

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Mechanical Loading of iMSC^SCX+ in a 2D Bioreactor for Cyclic Stretching

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주기적 스트레칭을 위한 2D 바이오리액터에서 iMSC^SCX+ 의 기계적 로딩

Harnessing iPSC-Derived iTenocytes for Effective Cell-Based Therapy

DOI:

10.3791/200708-v

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03:07 min

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March 01, 2024

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Victoria Yu^2,3, Angela Papalamprou^2,3, Dmitriy Sheyn^2,3,4,5

¹Orthopaedic Stem Cell Research Laboratory,Cedars-Sinai Medical Center, ²Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, ³Department of Biomedical Sciences,Cedars-Sinai Medical Center, ⁴Department of Orthopedics,Cedars-Sinai Medical Center, ⁵Department of Surgery,Cedars-Sinai Medical Center

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Keywords: IMSCSCX+2D Bioreactor Cyclic Stretching Mechanical Loading Fibronectin Scleraxis-positive Induced Mesenchymal Stromal Cells Uniaxial Tension Sinusoidal Strain Cell Organization Phalloidin Staining Tenogenic Genes Collagen Deposition

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Generation of induced pluripotent stem cell-derived iTenocytes via combined scleraxis overexpression and 2D Uniaxial tension(공막 과발현 및 2D 단축 장력 결합을 통한 유도만능줄기세포 유래 iTenocytes 생성)

주기적 스트레칭을 위한 2D 바이오리액터에서 iMSC^SCX+ 의 기계적 로딩

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