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Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
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Biology
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JoVE Journal Biology
Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

DOI:
10.3791/261-v

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10:58 min

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August 22, 2007

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, , , , , ,
1Department of Biomedical Engineering,University of California, Irvine (UCI), 2Stem Cell Research Center,University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia,University of California, Irvine (UCI)

Chapters

  • 00:00Title
  • 00:01Introduction
  • 02:25PDMS Molding of the Neuron Device Using an SU-8 Master
  • 04:15Cutting out the Microfluidic Neuron Device
  • 05:57Plasma Bonding the Device to Coverglass
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.

Tags

Microfluidic DeviceNeuron CulturingSoft LithographyPDMS FabricationSilicon WaferPhotolithographyMaster MoldNegative MoldReservoirsCover Glass BondingSterilization

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