The "Brain Milking" Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats

Dimitrios Dimitrakopoulos; Chrisitna Dimitriou; Freyja McClenahan; Robin J. M. Franklin; Ilias Kazanis

doi:10.3791/65308

Journal

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Neuroscience

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The “Brain Milking” Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats

JoVE Journal
Neuroscience
Author Produced

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JoVE Journal Neuroscience

The “Brain Milking” Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats

1,910 Views

•

06:52 min

•

February 09, 2024

DOI:

10.3791/65308-v

DOI:

10.3791/65308-v

•

06:52 min

February 09, 2024

•

1901 Views

Dimitrios Dimitrakopoulos¹, Chrisitna Dimitriou¹, Freyja McClenahan², Robin J. M. Franklin^2,3, Ilias Kazanis¹

¹Laboratory of Developmental Biology, Department of Biology,University of Patras ²Wellcome Trust-MRC Cambridge Stem Cell Institute,University of Cambridge ³Altos Labs,Cambridge Institute of Science

Summary

A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being.