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Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain
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Neuroscience
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JoVE Journal Neuroscience
Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

DOI:
10.3791/50880-v

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11:27 min

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November 18, 2013

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,
1Department of Medicine,University of Dresden, 2Center for Regerative Therapies Dresden

Chapters

  • 00:05Title
  • 01:47Culture Dish Preparation
  • 03:18Dissection, Plating, and Culturing
  • 06:35Neural Stem Cell Proliferation and Differentiation
  • 07:52Induction of Differentiation and Immunocytochemical Detection
  • 10:37Conclusion

Summary

Automatic Translation

Automatic Translation

Neural stem cells harvested from the adult brain are increasing utilized in applications ranging from the basic research of nervous system development to exploring potential clinical applications in regenerative medicine. This makes rigorous control in the isolation and culturing conditions used to grow these cells critical to sound experimental outcomes.

Tags

Neural Stem CellsAdult BrainCentral Nervous SystemStem Cell NicheNeural Progenitor CellsNeural DifferentiationNeurodegenerative ProcessesCNS RegenerationIn Vitro CultureNeural Stem Cell IsolationNeural Stem Cell MaintenanceNeural Stem Cell Differentiation

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