Obtaining Horizontal Mouse Hippocampal Brain Slices

Take the mouse brain hemispheres in a high-sucrose solution. Secure the hemispheres with their ventral side facing up on a vibratome specimen plate using an adhesive.

Transfer the specimen plate into the slicing chamber.

Add a semi-frozen high-sucrose solution to maintain the tissue's physiological conditions and solidify the adhesive.

Supply a carbon dioxide-oxygen mixture to maintain tissue oxygenation.

Using set parameters, obtain the initial slices and remove them. Further, obtain horizontal hippocampal slices.

Carefully transfer these brain slices into a chamber containing ACSF for tissue recovery.

The horizontal hippocampal slices are ready for further experimentation.

Place both hemispheres on the freshly-cut dorsal side with the ventral part of the brain facing up, and place a drop of superglue onto a specimen plate. Use a pipette tip to spread the glue into a big area to accommodate both pieces of tissue, and touch a piece of filter paper strap to the ventral side of one hemisphere. Use another filter paper strap to carefully semi-dry the dorsal side of the brain, and position the hemisphere dorsal side down onto the glue on the specimen plate.

Use two additional filter pa