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Immunology and Infection
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Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

DOI:
10.3791/3122-v

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10:09 min

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January 03, 2012

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,
1Centre for Immunity, Infection and Evolution,University of Edinburgh

Chapters

  • 00:05Title
  • 01:14Preparation and Routine Culture of Plasmodium falciparum
  • 02:54Routine Culture of Endothelial Cells and Preparation for Selection
  • 04:00Selection of P. falciparum for Cytoadhesion to Endothelial Cells
  • 07:36Increased Binding of Parasites to HBEC-5i Cells after Selection
  • 09:43Conclusion

Summary

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Automatic Translation

An in vitro model for cerebral malaria sequestration is described1. Plasmodium falciparum infected red blood cells are selected for binding to immortalized human brain microvascular endothelial cells. The selected parasites show a distinct phenotype. The selection process can be applied using various P. falciparum strains and endothelial cell lines.

Tags

Plasmodium FalciparumCytoadhesionHuman Brain Endothelial CellsCerebral MalariaMicrovasculatureSequestrationBlood Vessel WallsHBEC-5iIn Vitro ModelPanning AssayHigh-binding ParasitesPigmented Trophozoite Stage

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