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Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
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Medicine
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JoVE Journal Medicine
Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model

DOI:
10.3791/60450-v

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10:03 min

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November 21, 2019

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, , ,
1Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University and National Health Research Institutes, 2Center for Neurotrauma and Neuroregeneration,Taipei Medical University, 3TMU Neuroscience Research Center,Taipei Medical University, 4Department of Neurosurgery,Taipei Medical University Hospital, 5Department of Surgery, School of Medicine, College of Medicine,Taipei Medical University

Chapters

  • 00:05Title
  • 01:06Superparamagnetic Iron Oxide (SPIO) nanoParticles labeling of Mesenchymal Stem Cells (MSC)
  • 01:44Controlled Cortical Impact (CCI) Injury
  • 04:04Intranasal MSC Delivery
  • 05:57In Vivo Magnetic Resonance Imaging (MRI)
  • 06:54MSC Tracking and Quantification
  • 08:03Results: Representative SPIO-Labeled MSC Detection and Tracking
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for non-invasive mesenchymal stem cell (MSC) delivery and tracking in a mouse model of traumatic brain injury. Superparamagnetic iron oxide nanoparticles are employed as a magnetic resonance imaging (MRI) probe for MSC labeling and non-invasive in vivo tracking following intranasal delivery using real-time MRI.

Tags

Keywords: Superparamagnetic Iron OxideMesenchymal Stem CellsMRIIntranasal DeliveryTraumatic Brain InjuryMurine ModelNon-invasive TrackingTherapeutic EffectsCell LabelingControlled Cortical Impact InjuryStereotactic Frame

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