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Single Read und Paired End mRNA-Seq Illumina Bibliotheken von 10 Nanogramm Gesamt-RNA
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Single Read und Paired End mRNA-Seq Illumina Bibliotheken von 10 Nanogramm Gesamt-RNA

Article DOI: 10.3791/3340-v • 14:50 min • October 27th, 2011
October 27th, 2011 •
1, 1, 1, 1, 1,2,3, 1, 1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

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Hier beschreiben wir ein Verfahren zur Herstellung der beiden einzigen gelesen und gepaart Ende Illumina mRNA-Seq-Sequenzierung Bibliotheken zur Genexpressionsanalyse auf T7 lineare RNA-Amplifikation basieren. Dieses Protokoll erfordert nur 10 Nanogramm ab Gesamt-RNA und erzeugt sehr konsistent Bibliotheken für ganze Transkripte.

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Molecular Biology Ausgabe 56 Genetik mRNA-Seq Illumina-Seq Genexpressions-Profiling Hochdurchsatz-Sequenzierung
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