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Journal / Biology / Assaying the Kinase Activity of LRRK2 in vitro
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Assaying the Kinase Activity of LRRK2 in vitro
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Assaying the Kinase Activity of LRRK2 in vitro

Article DOI: 10.3791/3495-v • 06:10 min • January 18th, 2012
January 18th, 2012 •
1
1Department of Molecular Neuroscience, UCL Institute of Neurology

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Leucine Rich Repeat Kinase 2 is a large multidomain kinase, mutations in which are the most common genetic cause of Parkinson's disease. Analysis of the kinase activity of this protein has proven to be a crucial tool in understanding the biology and dysfunction of this protein. In this paper, in vitro assaying of the kinase activity of LRRK2 and a selection of its mutants is described, providing an experimental system to examine phosphorylation of putative substrates and potential dysfunction of LRRK2 in disease.

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Assaying Kinase Activity LRRK2 In Vitro Leucine Rich Repeat Kinase 2 ROCO Family Of Proteins GTPase Domain Kinase Domain Parkinson's Disease Enzymatic Activities Cell Death Mutations Inhibitors Experimental Tool Enzymatic Properties Drug Targets Approaches Epitope Tagged Protein Mammalian Cell Lines Immunoprecipitated
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