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Biochemistry

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JoVE Journal Biochemistry
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
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Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Article DOI: 10.3791/56688-v • 08:55 min • December 14th, 2017
December 14th, 2017 •
1, 1,2
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo

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Summary
Automatic Translation

The present study describes a simple method of detecting endogenous levels of Rab10 phosphorylation by leucine-rich repeat kinase 2.

Tags

Rab10 Phosphorylation LRRK2 Activity SDS-PAGE Phosphate-binding Tag Parkinson Research Disease Mutations Stoichiometry Western Blot Transfected HEK293 Cells DPBS Lysis Buffer Cell Lysate Microfuge Tubes Centrifugation Supernatants Protein Concentrations Bradford Assay
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