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JoVE Journal
Biochemistry

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Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
 

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Article doi: 10.3791/59037
February 21st, 2019

Summary February 21st, 2019

The objective of this protocol is to label, enrich, and identify substrates of protein kinase CK2 from a complex biological sample such as a cell lysate or tissue homogenate. This method leverages unique aspects of CK2 biology for this purpose.

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