/
/
Bestemmelse af fagocyterende Aktivitet of Clinical Antibody prøver
This content is Free Access.
Chapters
- 00:05Title
- 02:01Preparing Biotinylated Antigen
- 02:58Preparing Antigen Saturated Beads
- 04:08Preparing Antibody Samples and Plating the Experiment
- 05:44Flow Cytometric Analysis
- 06:29Antigen-saturated Beads and Phagocytosed in an Antibody-dependent Manner
- 08:24Conclusion
Vi præsenterer en high-throughput flowcytometrisk analyse for at bestemme fagocyterende aktivitet af antigen-specifikke antistoffer fra kliniske prøver, at udnytte fluorescerende antigen-belagt perler og en monocytic cellelinie udtrykker flere Fc receptorer-leverandører receptor brug og fagocyterende aktivitet bestemmelser på en standardiseret og reproducerbare mode for enhver antigen af interesse.
Tags
Phagocytic ActivityClinical Antibody SamplesFc ReceptorsClearancePathology Of DiseaseVariable DomainsInnate Immune CellsRecombinant Monoclonal Antibody TherapyNatural InfectionVaccinationIgG SubclassGlycosylationFunctional DifferencesAntigen-specific AntibodiesDisease StateSusceptibility To InfectionProgressionClinical OutcomeEffector FunctionEnhance InfectionFc Receptor-driven PhagocytosisImmune ComplexesTh1/Th2 Polarization Assay
