Journal
/
Biology
/
Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

DOI:
10.3791/56512-v

•

12:49 min

•

October 30, 2017

•

, ,
1Department of Biochemistry and Molecular Biology,University of Nebraska Medical Center, 2Department of Biomedical Sciences,GA-PCOM

Chapters

  • 00:05Title
  • 00:57Sample Procurement and Processing
  • 02:29Immunohistochemistry
  • 05:02Hematoxylin and Eosin Staining
  • 06:19Ductal Analysis
  • 08:33Immunohistochemictry Analysis
  • 09:43Results: Whole Mouse Mammary Gland Isolation and Analysis
  • 11:23Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the isolation of whole, intact mouse mammary glands to investigate extracellular matrix (ECM) expression and ductal morphology. Mouse #4 abdominal glands were extracted from 8-10 week old female nulliparous mice, fixed in neutral buffered formalin, sectioned and stained using immunohistochemistry for ECM proteins.

Tags

Keywords: Mammary GlandWhole MountExtracellular MatrixGland MorphologyTumor BiologyStromal RemodelingGene ExpressionProtein ExpressionDuctal ArchitectureNulliparous MiceFormalin FixationParaffin EmbeddingAntigen RetrievalImmunohistochemistry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56512?article_type=v

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
check_url/56512?article_type=v

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats
check_url/56512?article_type=v

Isolation of Mouse Salivary Gland Stem Cells
check_url/56512?article_type=v

Evaluation of Mammary Gland Development and Function in Mouse Models
check_url/56512?article_type=v

Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea
check_url/56512?article_type=v

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
check_url/56512?article_type=v

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands
check_url/56512?article_type=v

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
check_url/56512?article_type=v

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
check_url/56512?article_type=v

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Read Article