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Immunology and Infection

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JoVE Journal Immunology and Infection
Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
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Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

Article DOI: 10.3791/4454 • 11:49 min • May 2nd, 2013
May 2nd, 2013 •
1, 1, 1
1Department of Surgery, University of Wisconsin-Madison, School of Medicine and Public Health

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Summary
Automatic Translation

We describe a valuable diagnostic assay that could potentially be used to decide the withdrawal of immunosuppression after transplant without elevated risk of graft rejection. The assay uses the principles of Delayed Type Hypersensitivity and provides accurate assessment of both donor specific effector and regulatory immune responses mounted by recipients.

Tags

Trans-vivo Delayed-type Hypersensitivity Assay Antigen-specific Regulation T Cell-dependent Immune Response Foreign Antigen Sensitization Phase Challenge Phase Anergy Traditional DTH Assay Microbial Infections Transplant Patients Direct DTH Diagnostic Technique Human-to-mouse Transfer DTH Assay Peripheral Blood Mononuclear Cells (PBMCs) Specific Antigens Naïve Mouse Swelling Measurement Antigen Presentation Macrophages DCs Inflammatory Cascade
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