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Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
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Neuroscience
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JoVE Journal Neuroscience
Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

DOI:
10.3791/50641-v

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09:56 min

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July 27, 2014

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, , , ,
1Veterans Administration Medical Center, San Diego, 2Department of Neurosciences,University of California, San Diego

Chapters

  • 00:05Title
  • 01:51T3 Spinal Cord Transection
  • 04:25Preparation of Spinal Cord Neural Stem Cells
  • 06:22Transplantation of Cells into Spinal Cord Lesion
  • 07:29Results: Grafted Cell Survival and Morphology
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

Fibrin matrices containing growth factors were used to retain grafted neural stem cells into sites of complete spinal cord transection. Grafted cells completely filled the lesion cavity and differentiated into multiple neural cell types, including neurons that extended axons into host spinal cord over long distances.

Tags

Keywords: Neural Stem CellsSpinal Cord InjuryFibrinGrowth FactorsDifferentiationNeuronGliaGraft SurvivalAxon ExtensionTransplantation

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