Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

Christophe Bontoux; Virginie Lespinet-Fabre; Olivier Bordone; Virginie Tanga; Maryline Allegra; Myriam Salah; Salom&#233; Lalv&#233;e; Samantha Goffinet; Jonathan Benzaquen; Charles-Hugo Marquette; Marius Ili&#233;; V&#233;ronique Hofman; Paul Hofman

doi:10.3791/65190

Journal

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Biology

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Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

JoVE Journal
Biology

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JoVE Journal Biology

Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

1,039 Views

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07:59 min

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September 08, 2023

DOI:

10.3791/65190-v

DOI:

10.3791/65190-v

•

07:59 min

September 08, 2023

•

1029 Views

Christophe Bontoux^1,2,3,4,5, Virginie Lespinet-Fabre^1,2,3,4, Olivier Bordone^1,2,3,4, Virginie Tanga^1,2,3,4, Maryline Allegra^1,2,3,4, Myriam Salah^1,2,3,4, Salomé Lalvée^1,2,3,4, Samantha Goffinet^1,2,3,4, Jonathan Benzaquen^3,4,5,6, Charles-Hugo Marquette^3,4,5,6, Marius Ilié^1,2,3,4,5, Véronique Hofman^1,2,3,4,5, Paul Hofman^1,2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d’Azur, Hôpital Pasteur, CHU de Nice ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d’Azur, Hôpital Pasteur, CHU de Nice ⁴FHU OncoAge,Université Côte d’Azur ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d’Azur ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur

Summary

The increase of molecular biomarkers to be tested for non-squamous non-small cell lung cancer (NS-NSCLC) care management has prompted the development of fast and reliable molecular detection methods. We describe a workflow for genomic alteration assessment for NS-NSCLC patients using an ultra-fast-next generation sequencing (NGS) approach.