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Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
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Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Article DOI: 10.3791/62791-v • 14:34 min • December 25th, 2021
December 25th, 2021 •
*1, *1, 1, 1, 1, 1, 2, 1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
* These authors contributed equally

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Here we present, a method to visualize ternary complex formation between three protein partners using fluorescent-tagged proteins by BiFC based FRET-FLIM assay. This method is valuable for studying protein-protein interaction complexes in vivo.

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Protein-protein Interactions MADS-box Transcription Factor Calcium Sensor Protein BiFC-FRET-FLIM Assay Fluorescence Lifetime Measurements Tripartite Interaction Homodimerize Cytoplasm Split YFP Proteins FRET Acceptor FRET Donor Amplification Of Coding Sequences PCR Cloning Entry Vectors Antibiotic Selection Restriction Digest DNA Sequencing Destination Vectors
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