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A Protocol for Analyzing Hepatitis C Virus Replication
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

DOI:
10.3791/51362-v

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13:04 min

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June 26, 2014

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, ,
1Liver Program at Regenerative Medicine Institute, Department of Biomedical Sciences, Department of Surgery,Cedars-Sinai Medical Center, 2Department of Surgery,David Geffen School of Medicine at UCLA

Chapters

  • 00:05Title
  • 02:06In Vitro HCV RNA Transcription
  • 03:24HCV RNA Transfection and Sample Collection
  • 05:42RT-qPCR for Assessing HCV Genome Copies and Western Blot Analysis for Detecting HCV Protein Expression
  • 07:31Immunofluorescence Assay
  • 08:52Measuring Virus Titer
  • 09:56Results: Analysis of Hepatitis C Virus Replication
  • 11:50Conclusion

Summary

Automatic Translation

Automatic Translation

Hepatitis C Virus (HCV) is a major human pathogen that causes liver disorders, including cirrhosis and cancer. An HCV infectious cell culture system is essential for understanding the molecular mechanism of HCV replication and developing new therapeutic approaches. Here we describe a protocol to investigate various stages of the HCV replication cycle.

Tags

Keywords: Hepatitis C VirusHCVFlaviviridaeViral ReplicationCell Culture SystemHuh-7 CellsQuantitative RT-PCRDouble-stranded RNAWestern BlotImmunofluorescenceLuciferase AssayViral EntryGenome ReplicationPackagingEgress

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