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JoVE Journal
Biochemistry

This content is Open Access.

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
 

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Article doi: 10.3791/60970
June 8th, 2020

Summary June 8th, 2020

We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.

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