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Journal / Biochemistry / TMT Sample Preparation for Proteomics Facility…
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Biochemistry

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JoVE Journal Biochemistry
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Article DOI: 10.3791/60970-v • 07:44 min • June 8th, 2020
June 8th, 2020 •
1, 1, 1, 1
1Department of Pediatrics, University of Florida

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We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.

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TMT Sample Preparation Proteomics Facility Submission Data Analysis Labeling Cost Reduction Protein Extraction Improvement High-quality Data Generation Labeling Efficiencies Reproducibility Reliable Data Acquisition Muscle Tissue Isolation Lysate Creation Bead Disruptor Sonication Centrifugation Reducing/alkylating Reagent Treatment TCEP Incubation
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