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Quantification of Orofacial Phenotypes in Xenopus
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JoVE Journal Biology
Quantification of Orofacial Phenotypes in Xenopus

Quantification of Orofacial Phenotypes in Xenopus

DOI:
10.3791/52062-v

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09:26 min

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November 06, 2014

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,
1Biology Department,Virginia Commonwealth University

Chapters

  • 00:05Title
  • 01:04Preparing the Head Mounting Dish
  • 01:58Photographing the Orofacial Region
  • 03:34Analyzing the Facial Dimensions
  • 04:23Quantitative Analysis and Morphometrics
  • 07:55Results: Orofacial Morphology after Retenoic Acid Receptor Inhibitor Treatment
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

A method to quantify the orofacial size and shape of Xenopus laevis embryos has been developed. In this protocol, traditional size measurements are combined with geometric morphometrics to allow for more sophisticated analyses of orofacial development and defects.

Tags

XenopusOrofacial PhenotypesCraniofacial DevelopmentQuantification2D ImagingSize DimensionsOrofacial WidthHeightAreaMouth OpeningRoundnessGeometric MorphometricsLandmarksPrincipal Component AnalysisDiscriminant Function AnalysisTransformation GridWarping Pattern

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