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Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
 

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Article DOI: 10.3791/1949
May 23rd, 2010

Chapters

Summary May 23rd, 2010

Xenopus embryonic epithelia are an ideal model system to study cell behaviors such as polarity development and shape change during epithelial morphogenesis. Traditional histology of fixed samples is increasingly being complemented by live-cell confocal imaging. Here we demonstrate methods to isolate frog tissues and visualize live epithelial cells and their cytoskeleton using live-cell confocal microscopy.

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