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Developmental Biology

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Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
 

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Article DOI: 10.3791/56802-v 12:46 min December 6th, 2017
December 6th, 2017

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Summary

This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.

Tags

Keywords: Fluorescence In Situ Hybridization (FISH) Drosophila Oocytes Prometaphase I Metaphase I Meiotic Arm Cohesion Maternal Age Effect Oocyte Rolling PBSBTX Frosted Glass Slides
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