Journal
/
Developmental Biology
/
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

DOI:
10.3791/56802-v

•

12:46 min

•

December 06, 2017

•

,
1Department of Biological Sciences,Dartmouth College

Chapters

  • 00:05Title
  • 00:50Removal of Chorions and Vitelline Membranes
  • 05:57FISH
  • 10:27Results: Representative Images of Meiotic Figures with Intact Arm Cohesion and Disrupted Arm Cohesion
  • 11:31Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.

Tags

Keywords: Fluorescence In Situ Hybridization (FISH)Drosophila OocytesPrometaphase IMetaphase IMeiotic Arm CohesionMaternal Age EffectOocyte RollingPBSBTXFrosted Glass Slides

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56802?article_type=v

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
check_url/56802?article_type=v

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
check_url/56802?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/56802?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
check_url/56802?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/56802?article_type=v

Wholemount In Situ Hybridization for Astyanax Embryos
check_url/56802?article_type=v

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
check_url/56802?article_type=v

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
check_url/56802?article_type=v

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Read Article