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Biology

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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
 

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Article DOI: 10.3791/4400
December 10th, 2012

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Summary December 10th, 2012

A quantitative method for the analysis of chromosome replication timing is described. The method utilizes BrdU incorporation in combination with fluorescent in situ hybridization (FISH) to assess replication timing of mammalian chromosomes. This technique allows for the direct comparison of rearranged and un-rearranged chromosomes within the same cell.

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