Journal
/
Biology
/
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

DOI:
10.3791/4400-v

•

17:14 min

•

December 10, 2012

•

,
1Department of Biochemistry and Molecular Biology, Knight Cancer Institute,Oregon Health & Science University

Chapters

  • 00:05Title
  • 01:53BrdU Incorporation: Terminal Labeling
  • 02:35Chromosome Harvest of Monolayer Cells Cultures
  • 05:53RNase Treatment and Ethanol Dehydration
  • 06:38Fluorescent Labeling BAC DNA: Nick Translation
  • 08:19In situ Hybridization
  • 12:18Capturing Images and Quantifying BrdU Incorporation
  • 14:27Results: Chromosome Replication Timing and FISH
  • 16:45Conclusion

Summary

Automatic Translation

Automatic Translation

A quantitative method for the analysis of chromosome replication timing is described. The method utilizes BrdU incorporation in combination with fluorescent in situ hybridization (FISH) to assess replication timing of mammalian chromosomes. This technique allows for the direct comparison of rearranged and un-rearranged chromosomes within the same cell.

Tags

Chromosome Replicating TimingFluorescent In Situ HybridizationDNA ReplicationS PhaseTemporal Replication ProgramDevelopmental CuesEpigenetic ModificationsX InactivationTranscriptional SilencingX Inactivation CenterChromosome RearrangementsCancer CellsIonizing Radiation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4400?article_type=v

Double Whole Mount in situ Hybridization of Early Chick Embryos
check_url/4400?article_type=v

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
check_url/4400?article_type=v

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
check_url/4400?article_type=v

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
check_url/4400?article_type=v

In Situ Hybridization for the Precise Localization of Transcripts in Plants
check_url/4400?article_type=v

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
check_url/4400?article_type=v

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
check_url/4400?article_type=v

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)
check_url/4400?article_type=v

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
check_url/4400?article_type=v

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Read Article