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Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
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Biology
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JoVE Journal Biology
Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

DOI:
10.3791/52918-v

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08:00 min

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July 19, 2015

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, , ,
1Department of Medicine,Duke University Medical Center, 2Department of Chemistry,University of North Carolina – Chapel Hill, 3Renovo Neural Incorporated

Chapters

  • 00:05Title
  • 01:34Harvesting, Dissecting, and Fixing Tissue
  • 02:50Infiltrating and Embedding Tissue Sections in Resin
  • 04:17Optimizing SBEM Images for Surface Segmentation
  • 05:33Results: Correlative Confocal and SEM Analysis of Epithelial Cell Ultrastructure
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.

Tags

Keywords: Correlative MicroscopyConfocal Microscopy3D Electron MicroscopyEnteroendocrine CellsIntestinal EpitheliumTransgenic Reporter MicePeptide YY-GFPUltrastructureVolume Rendering

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