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Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
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JoVE Journal Neuroscience
Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

DOI:
10.3791/58027-v

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09:55 min

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September 05, 2018

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, , ,
1RIKEN Brain Science Institute, 2Tokyo Metropolitan Institute of Medical Science

Chapters

  • 00:04Title
  • 00:42Tracer Injection
  • 03:32Tissue Collection
  • 04:23Clearing with Antibody Staining
  • 08:00Results: Large-scale Imaging and High Magnification Photographs
  • 09:19Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a procedure for tissue clearing, fluorescent labeling, and large-scale imaging of mouse brain tissue which, thereby, enables visualization of the three-dimensional organization of cell types in the neocortex.

Tags

Keywords: 3D ImagingCellular OrganizationMouse NeocortexNeuroanatomyNeural TracingCholera Toxin Subunit BStereotaxic Injection

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