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Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
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Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

DOI:
10.3791/53318-v

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09:02 min

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December 10, 2015

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, ,
1Department of Ophthalmology and Visual Sciences,Washington University in St. Louis School of Medicine

Chapters

  • 00:05Title
  • 01:24Preparation of Mice and Materials for Dissection and Tissue Collection
  • 02:06Dissection and Tissue Collection
  • 02:38Tissue Processing and Sectioning
  • 04:06Staining and Imaging of Cerebellum
  • 06:41Results: Disruption of Endogenous LINC Complexes in Cerebellar Purkinje Cells
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

Nuclear envelope proteins play a central role in many basic biological processes and have been implicated in a variety of human diseases. This protocol describes a new Cre/Lox-based mouse model that allows for the spatiotemporal control of LINC complexes disruption.

Tags

Keywords: LINC ComplexesNuclear MigrationNuclear AnchorageSun ProteinsNesprinsDominant-negative Transgenic MouseCre/Lox SystemCell-type Specific Disruption

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