Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Eva Porlan; Beatriz Mart&#237;-Prado; Antonella Consiglio; Isabel Fari&#241;as

doi:10.3791/53282

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of N...

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Developmental Biology

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JoVE Journal Developmental Biology

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

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08:48 min

February 17, 2016

DOI: 10.3791/53282-v

Eva Porlan¹, Beatriz Martí-Prado^2,3, Antonella Consiglio^4,5, Isabel Fariñas^2,3

¹Cell Division and Cancer Group,Spanish National Cancer Research Centre (CNIO), ²Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ³Departmento de Biologìa Celular,Universidad de Valencia, ⁴Institut de Biomedicina de la Universitat de Barcelona (IBUB), ⁵Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit,University of Brescia

Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.

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Genetic Modification Neural Stem Cells Adult Mouse V-SVZ Lentiviral Vectors Ependymal Cells Cell Cycle-independent Infection Fluorescent Proteins Cell Autonomous And Non-autonomous Effects