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JoVE Journal Developmental Biology
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Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Article DOI: 10.3791/53282 • 08:48 min • February 17th, 2016
February 17th, 2016 •
1, 2,3, 4,5, 2,3
1Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), 2Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 3Departmento de Biologìa Celular, Universidad de Valencia, 4Institut de Biomedicina de la Universitat de Barcelona (IBUB), 5Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia

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Summary
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Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.

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Genetic Modification Cells Adult Mouse V-SVZ Analysis Neural Stem Cells Autonomous Effects Non-autonomous Effects Lentiviral Particles DNA Constructs Ventricular-subventricular Zone Ependymal Cells Cell Types Interactions Niche Lentiviral Vectors Genome Integration Cell Cycle Independent Long-term Diffusion Grad Student Beatriz Martínez Quiescent Somatic Stem Cells Cell Renewal Adult Tissues Neurogenic Niches Subgranular Zone Dentate Gyrus Hippocampus Subependymal Zone SEZ Walls Of The Lateral Ventricles In Vivo Gene Transfer Strategies
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