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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

DOI:
10.3791/56889-v

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07:53 min

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January 01, 2018

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, ,
1Berlin Institute for Medical Systems Biology,Max Delbrück Center for Molecular Medicine in the Helmholtz Association

Chapters

  • 00:05Title
  • 01:15Preparation of RNAi Plates
  • 02:37Preparation of C. elegans Strain BAT28
  • 04:56Induction of Germ Cell Reprogramming
  • 06:01Results: Assessing Reprogrammed Cells
  • 06:52Conclusion

Summary

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This protocol describes how to study cellular processes during cell fate conversion in Caenorhabditis elegans in vivo. Using transgenic animals, allowing heat-shock promoter-driven overexpression of the neuron fate-inducing transcription factor CHE-1 and RNAi-mediated depletion of the chromatin-regulating factor LIN-53 germ cell to neuron reprogramming can be observed in vivo.

Tags

Keywords: RNAiHeat-shockTranscription FactorGerm CellsNeuronsC. ElegansCellular ReprogrammingRegenerative TherapyGene ExpressionDevelopmental BiologyEpigeneticsSignaling PathwaysIn VivoGenetic ScreenLin-53BAT28 StrainAge SynchronizationBleaching

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