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JoVE Journal
Bioengineering

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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
 

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Article DOI: 10.3791/53564
April 6th, 2016

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Summary April 6th, 2016

A quick protocol for proteolytic digestion with an in-house built flow-through tryptic microreactor coupled to an electrospray ionization (ESI) mass spectrometer is presented. The fabrication of the microreactor, the experimental setup and the data acquisition process are described.

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