Journal
/
Biology
/
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

DOI:
10.3791/62554-v

•

08:04 min

•

May 11, 2021

•

, , ,
1Einthoven Laboratory for Experimental Vascular Medicine, Department of Internal Medicine (Nephrology),Leiden University Medical Center, 2Institute Physics for Medicine Paris,INSERM U1273, ESPCI Paris, CNRS FRE 2031, 3MEMOLIFE Laboratory of Excellence and PSL Research University

Chapters

  • 00:04Introduction
  • 01:14Embryoid Bodies (EB) Formation in Hanging Drop
  • 03:02Floating EBs Culture for Vascular Differentiation
  • 03:413D Sprouting Angiogenesis Assay and Immunofluorescence Staining
  • 05:44Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

This assay utilizes mouse embryonic stem cells differentiated into embryoid bodies cultured in 3D-collagen gel to analyze the biological processes that control sprouting angiogenesis in vitro. The technique can be applied for testing drugs, modeling diseases, and for studying specific genes in the context of deletions that are embryonically lethal.

Tags

Keywords: In VitroSprouting AngiogenesisMouse Embryonic Stem CellsVascular Disease ModelingDrug TestingEndothelial Tip CellsCell MigrationGenetic ScreeningPluripotencyKaryotypingMesoderm DifferentiationLow Attachment Polystyrene Dishes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62554?article_type=v

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
check_url/62554?article_type=v

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
check_url/62554?article_type=v

Isolation and Derivation of Mouse Embryonic Germinal Cells
check_url/62554?article_type=v

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
check_url/62554?article_type=v

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
check_url/62554?article_type=v

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
check_url/62554?article_type=v

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
check_url/62554?article_type=v

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
check_url/62554?article_type=v

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
check_url/62554?article_type=v

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Read Article