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1Molecular Imaging and Neurovascular Research Laboratory,Dongguk University College of Medicine, 2Biomedical Research Center,Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering,Seoul National University, 4Departments of Radiology and Cancer Systems Imaging,University of Texas M.D. Anderson Cancer Center
Chapters
- 00:05Title
- 01:20Exogenously Formed Clot Preparation and Fluorescent Marker Labeling
- 02:55Thromboembolic Stroke Model Preparation
- 05:42In Vivo MicroCT Cerebral Thrombus Imaging
- 06:36Ex Vivo Near Infrared Fluorescent (NIRF) Thrombus Imaging and MicroCT Thrombus Area Imaging Quantification
- 09:48Results: Representative Direct Cerebral Thrombus Imaging before and after Thrombolysis Activation
- 11:28Conclusion
This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.
