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Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
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Neuroscience
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JoVE Journal Neuroscience
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

DOI:
10.3791/54608-v

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08:44 min

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October 13, 2016

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, , ,
1Department of Ophthalmology,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:03Retina Dissection
  • 03:30Whole-cell Patch Clamp Recording from Flat-mount Retina
  • 06:34Results: Morphology and Membrane Potentials of ON-and Off-Starburst Amacrine Cell in the Nob Mouse Retina
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates how to perform whole-cell patch clamp recording on retinal neurons from a flat-mount preparation.

Tags

Keywords: Patch ClampStarburst Amacrine CellsFlat-mountDeafferentated Mouse RetinaRetinal NeurobiologyRetinal CircuitsWhole-cell RecordingNitrocellulose MembraneExternal SolutionCarbogenatedUpright Microscope

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