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Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
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Neuroscience
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JoVE Journal Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

DOI:
10.3791/50400-v

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11:46 min

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May 16, 2013

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, ,
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney , 2The MARCS Institute,University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney

Chapters

  • 00:05Title
  • 02:05General Set Up and Tissue Preparation
  • 05:32Patching Cell Bodies of Retinal Ganglion Cells
  • 07:20Recordings of Ganglion Cells Using Dynamic Clamp
  • 09:12Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
  • 10:14Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
  • 11:27Conclusion

Summary

Automatic Translation

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This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

Tags

Keywords: Dynamic ClampSynaptic ConductanceArtificial ConductanceRetinal Ganglion CellsPatch ClampVoltage ClampCurrent ClampNeuronal ExcitabilityExcitatory InputsInhibitory InputsAlpha Functions

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