Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Jin Y. Huang; Klaus M. Stiefel; Dario A. Protti

doi:10.3791/50400

Journal

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Neuroscience

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Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

12,234 Views

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11:46 min

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May 16, 2013

DOI:

10.3791/50400-v

DOI:

10.3791/50400-v

•

11:46 min

May 16, 2013

•

3 Views

Jin Y. Huang¹, Klaus M. Stiefel², Dario A. Protti³

¹Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney , ²The MARCS Institute,University of Western Sydney, ³Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney

Summary

This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.