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Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques
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Neuroscience
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JoVE Journal Neuroscience
Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

DOI:
10.3791/52422-v

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10:30 min

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February 11, 2015

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1Anatomy and Cell Biology,Wayne State University School of Medicine, 2Ophthalmology,Wayne State University School of Medicine

Chapters

  • 00:05Title
  • 01:19Retinal Dissection and Slice Preparation
  • 06:10Patch Clamp Recordings
  • 09:23Results: Light-evoked Postsynaptic Responses
  • 10:07Conclusion

Summary

Automatic Translation

Automatic Translation

We will demonstrate how to prepare retinal slices from the mouse eye and record light responses in retinal neurons. The entire procedure is conducted in dark-adapted conditions.

Tags

Keywords: RetinaVisual SystemNeural NetworkRetinal NeuronsBipolar CellsGanglion CellsAmacrine CellsPatch ClampDark-adaptedMouse Retinal SliceLight-evoked Synaptic ResponsesVisual Signal Processing

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