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Chapters
- 00:05Title
- 00:47Preparation of Media and Starter Cultures
- 01:54Co-inoculation of Fluorescently Labeled Strains for Surface Spreading
- 03:16Co-inoculation of Fluorescently Labeled Strains for Biofilm Growth
- 04:32Colony Imaging Using Fluorescent Microscopy
- 05:36Results: Swarming, Sliding, and Biofilm Producing Colonies Display Different Patternsof Spatial Growth
- 07:12Conclusion
The role of assortment (spatial segregation) in evolutionary scenarios can be examined using simple microbial systems in the laboratory that allow controlled adjustment of spatial distribution. By modifying the founder cell density, various assortment levels can be visualized using fluorescently labelled bacterial strains in the colony biofilms of Bacillus subtilis.
Tags
Keywords: Spatial SegregationMicrobial PopulationsFluorescent MicroscopySwarmingSlidingBiofilm FormationSocial MicrobiologyFitnessImage AnalysisB. SubtilisLB MediumSwarming PlatesSliding PlatesColony BiofilmSG MediumGreen And Red Fluorescent ProteinOptical DensityMicroscopic TechniquesMicrobial Interaction
