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Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

DOI:
10.3791/54968-v

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07:35 min

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December 24, 2016

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, ,
1Division of Hygiene and Medical Microbiology,Medical University of Innsbruck

Chapters

  • 00:05Title
  • 00:51Peripheral Blood Mononuclear Cell (PBMC) Enrichment
  • 02:30Monocytes Anti-human CD14 Magnetic Particle Isolation
  • 04:08Monocyte Interleukin (IL)-4 and Granulocyte Macrophage–Colony Stimulating Factor (GM-CSF) Stimulation
  • 05:10Results: Representative Human Monocyte–Dendritic Cell Generation from Whole Blood
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.

Tags

Keywords: MonocyteDendritic CellsMagnetic Beads SeparationCD14ImmunologyAntigen PresentationPBMCDensity Gradient CentrifugationMagnetic Particle Isolation

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