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JoVE Journal
Biology

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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
 

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Article DOI: 10.3791/773
July 9th, 2008

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Summary July 9th, 2008

Antigen presentation in secondary lymphoid organs by dendritic cells is crucial for the initiation of the T cell mediated adaptive immune response. Here we demonstrate the culture of bone marrow derived murine dendritic cells, activation, and labeling for 2-photon imaging.

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